[Histonet] Collagen staining using special stains, lighter vs darker

[Geoff McAuliffe]

The first things to check are 1. adequate fixation. 2. Complete removal 
of paraffin.
That done, I don't think you can use staining intensity to draw any 
conclusions about the health, or lack thereof, of collagen.
I suggest finding some references on collagen production and degradation 
before proceeding.

Geoff

Madary, Joseph wrote:
> I have an investigator looking at Massons(with Aniline Blue), Van Gieson
> and Gomori's all showing the same type of collagen staining.  In some
> areas collagen fibers are brilliantly stained and other fibers are pale
> all on the same slide.  This is consistent across the board so we know
> it is not the stain, rather the tissue response.  I want to say I have
> heard lighter collagen fibers are either immature or dying.  I mean that
> is fine, but does anyone have any real answers?
>
>  
>
> Nick Madary, HT/HTL(ASCP)QIHC
>
> Histology Mgr, Medimmune
>
> 301.398.6360(lab), 4745(vm),9745(fax)
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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