[Histonet] human macrophages in human melanoma xenografts

[Margaryan, Naira]

Thanks a lot both of you!

For melanoma detection, I usually use HRP with AEC. Is there any non-mouse anti-human macrophage marker?

Thank you much,
Naira 


-----Original Message-----
From: Liz Chlipala [mailto:liz <@t> premierlab.com] 
Sent: Friday, October 23, 2009 10:51 AM
To: Galbraith, Joe; Margaryan, Naira; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] human macrophages in human melanoma xenografts 

F4/80 is a mouse macrophage marker.  If she wants to detect human
macrophages in a mouse background she will need to use a mouse
anti-human CD68.  She will need to run it with a mouse on mouse
detection system and run all of the appropriate negative controls.  I
would also select a alkaline phosphatase detection system rather than
HRP with DAB just incase the tumor has any melanin in it.  I have done
this before looking for human lymphocytes with LCA in a mouse
background.  You just need to make sure you have all of the appropriate
controls.  For a positive control I would use a human tonsil or
something like that and you need to make sure you run the appropriate
isotype negative controls.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Galbraith, Joe
Sent: Friday, October 23, 2009 9:44 AM
To: Margaryan, Naira; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] human macrophages in human melanoma xenografts 

Naira:

Here is a link to a site listing macrophage markers.  F4/80 is a
commonly mentioned marker for macrophages.  I presume you mean IHC
rather than ICH.  Enjoy.

http://www.antibodybeyond.com/reviews/cell-markers/macrophage-marker.htm

Joe
joseph-galbraith <@t> uiowa.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Margaryan, Naira
Sent: Friday, October 23, 2009 10:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] human macrophages in human melanoma xenografts 

Hi Histonetters,

I would like to be able to look at human macrophages in human melanoma
xenografts raised in mouse. Could you please suggest me a best Ab for
ICH and protocol?



Thanks in advance,

Naira


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