[Histonet] RE: Antigen retrieval question

[Connolly, Brett M]

Perhaps reversal is not the right word...slides were left overnight in
buffer w/ tween. We got very minimal faint staining compared to previous
runs done in one day.

Brett 

-----Original Message-----
From: Morken, Tim [mailto:Timothy.Morken <@t> ucsfmedctr.org] 
Sent: Tuesday, October 20, 2009 2:29 PM
To: Connolly, Brett M; histonet <@t> lists.utsouthwestern.edu
Subject: RE: Antigen retrieval question

Brett, how long? I've left them overnight before proceeding without any
problems. 

It would not be "re-fixation" as with formalin. However, if it is in a
detergent buffer it might cause some other kind of denaturation.


Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Tuesday, October 20, 2009 11:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antigen retrieval question

Has anyone experienced a "reversal" of citrate HEIR unmasking do to a
prolonged delay in continuing the experiment after the retrieval step? 

Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly <@t> merck.com

  
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