[Histonet] need help in selecting blades for MicrotomeLeitz1512and good place for service

[Andrea Grantham]

I agree. Klaus is probably your best bet.




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

"happy slicing and dicing and may all your stains work perfectly" -  
Paula Sicurello
P Please consider the environment before printing this email.



On Oct 20, 2009, at 7:32 AM, Weems, Joyce wrote:

> And if you want to speak to someone who trained in the Leitz factory,
> Klaus Dern is in GA - Microprecision Instruments Corp. 706-635-8840.  
> He
> can probably build you one!!
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of  
> Armandi,
> Arlene
> Sent: Tuesday, October 20, 2009 10:15
> To: Bader Siddiki; Histonet
> Subject: RE: [Histonet] need help in selecting blades for
> MicrotomeLeitz1512and good place for service
>
> Hi Bader,
> I've been a Histotechnologist for 30 years, and the Leitz 1512 is the
> best microtome I have ever used. (And I've used a lot)I can easily cut
> 1-2 micron sections of any tissue, including bone.
> I don't know where you are located, but if you are anywhere near Los
> Angeles, "Fritz Instruments" serviced my three 1512s for years and
> always did an excellent job. They are great with older microtomes.
> As for disposable blades, the Tissue-Tek Accu-Edge blades are the best
> on the market.
> If you are looking for the knife holder that is part of the original
> instrument, try IMEB instruments, they carry used 1512s and probably
> have some spare parts. Ph# 800-543-8496. or imebinc.com If you are
> looking for the blade holder that fits into the 1512's knife holder,
> that is Tissue-Tek #4687. IMEB also carries that, as does EMS  
> ( Electron
> Microscopy Sciences)  Cat# 63048-10  800-523-58874 A couple of
> hints/suggestions for using the 1512...
> -When you trim your blocks, don't "rock" the handwheel, make complete
> rotations.
> -Oil the unit weekly (if you look under the top lid you should see  
> that
> the oil ports are marked.)
> - Lubricate the Teflon plate (black plate under the knife holder) at
> least weekly (more often if heavy use). Use microtome grease (not oil)
> for this.
> - I have always had to remove the knife guards on the unit to allow  
> for
> using a disposable blade holder. The units were originally made to be
> used with the old reusable "hard knives", thus they were made with the
> knife guards built in.
> Hope some of this helps. If you have any other questions about the
> 1512, please feel free to call or e-mail me.
>
>
>
> Arlene Armandi, HTL, HT(ASCP)
> Cedars-Sinai Medical Center
> Los Angeles, CA 90048
> 310-423-4756
> arlene.armandi <@t> cshs.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bader
> Siddiki
> Sent: Monday, October 19, 2009 3:37 PM
> To: Histonet
> Subject: [Histonet] need help in selecting blades for Microtome
> Leitz1512and good place for service
>
> Hello Histonets
> I need some help and advice from you experts.
> We are new in cutting formalin fixed paraffin embedded (FFPE) tissue
> sections.
> We need your advice.
> We have a used Leitz 1512 microtome.
> ------- perhaps it needs service, can you recommend a good and
> reasonable
> place.
> --------what  will be a good disposable blades to buy for cutting FFPE
> tissues sections (mostly soft tissues like colon, stomach, skin,  
> cancer
> tissues  etc).
> --------this  instrument needs a blade holder, what would you  
> recommend,
> Leitz does not sell blade holders for this instrument anymore.
> --------any good technique for cutting tissues for a person who is not
> expert.
> --------good place to buy formalin fixed tissue blocks for research  
> use.
>
> Thanks a million.
> Bader
>
> e-mail address: baderbo <@t> gmail.com
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