[Histonet] pkh

[Melissa Mazan]

Hi all,
We have collected a specific lung cell via FACS from mice, stained the 
cells with PKH, and then returned the cells to congenic mice via 1. 
tracheal injection or 2. tail vein injection.  We can see the PKH 
positive cells in the recipient lungs on microscopy, but we are having a 
real problem costaining these cells with anything else such as vimentin 
- has anyone else tried this approach? Melissa

-- 
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Equine Sports Medicine
Department of Clinical Sciences
Tufts Cummings School of Veterinary Medicine
200 Westborough Road
North Grafton,MA 01536
tel:	508-839-5395
fax:	508-839-7903
email:	melissa.mazan <@t> tufts.edu