[Histonet] Folds in tissue

[Clare Thornton]

We have been having ongoing issues with microscopic folds in all types of tissue.  On the waterbath, the section looks neat and wrinkle free.  Under the scope, there are many folds that are beginning to interfere with diagnosis.  We notice the folds mostly in skin, although they have appeared in practically every type of tissue.  We have been experimenting with water bath temperature;  cutting on 5 microns vs. 4;  baking upright in a rack or lying flat;  using tissue adhesive (we use Sta-On by Surgipath) or not; and various other factors.  Nothing has really jumped out as being a deciding factor.  Any hints, tips, tricks, or experiments to try?  What have others done when dealing with an issue like this?  Thanks for all your help.

Clare J. Thornton, HTL(ASCP)
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com