[Histonet] Embedding and Sectioning of tissue containing Gels.

[Melanie Black]

corrected a spelling error!

>
> Hi All
>
> We are looking at rat issue which has had a gel subcutaneously  
> injected into it. We have routinely processed it and embedded it  
> into wax. The gel appears to survive processing and embedding, but  
> then seems to be pulled out or distorted during sectioning. We  
> would like to visualize it in tact to look at cells within the gel.
>
> I have alternately embedded it in JB 4 resin (which is water  
> soluble and does not require dehydration. This method is so far the  
> best, however there are still some challenges.
> i) There are sometimes bubbles in the resin that occur during  
> polymerization.
> ii) Some gels, depending on formulations don't take up the resin  
> entirely, and therefore are still too soft to section.
>
> We use a glass knife to section this resin. Staining is good, and  
> immunofluorescence in the form of ED-1 and Actin are successful.
>
> Does anybody have any experience with this type of research related  
> problem?
>
> Many Thanks
> Melanie Black
>
> Melanie Black
> 082 469 3352
>
> Cardiovascular Research Unit
> 3rd Floor; Chris Barnard Building
> Medical School;
> Observatory. 7925.
> University of Cape Town.
> South Africa.
>
>
>

Melanie Black
082 469 3352

Cardiovascular Research Unit
3rd Floor; Chris Barnard Building
Medical School;
Observatory. 7925.
University of Cape Town.
South Africa.