[Histonet] frozen myocardium sections

[Geoff McAuliffe]

1. Cool the isopentane with liquid N. Put a metal rod (aluminum, brass, 
copper) in the chilled isopentane. Wait for the rod to cool.
2. Put the tissue+OCT on a metal object disk, microtome chuck or even a 
small metal plate.
3. Put the metal supporting the tissue on the chilled metal rod, the 
tissue will freeze rapidly without cracking or touching the isopentane.
Voila!
Put the tissue in the cryostat at the appropriate temperature for 
sectioning and have some coffee while the tissue "warms up" to cutting 
temperature.

Geoff


Jean-Martin Lapointe wrote:
> Hi all,
>
> for a study we are freezing myocardium sections in OCT immersed directly
> in liquid nitrogen, without isopentane, because apparently isopentane
> quenches the fluorescence of the cells we need to detect in the tissue.
> Unfortunately the blocks tend to crack after freezing. Does anyone have
> a suggestion to avoid cracking ?
>
> thanks
>
>  
>
> __________________________________
>
> Jean-Martin Lapointe
>
> AccelLAB Inc
>
> jm.lapointe <@t> accellab.com <mailto:jm.lapointe <@t> accellab.com> 
>
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>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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