[Histonet] Paraffin sectioning mouse embryos

[Sandra Moeller]

Dear Histonetters,

I hope you can help me. I'm working with mouse embryos. Now I have some
samples       (~ E.10.5), which have already gone through ISH. I tried to
make some paraffin sectionings from them, but most of the sectionings  were
compressed anddid't look very nice. I tried the processing with different
times and the slices in different thicknesses.
Younger embryos w/o ISH get very hard and break easily.
Hopefully one of you has an idea ;-)

Thank you very much,
Sandra