[Histonet] Sakura TDE 30

[louise renton]

OK,


Firstly, what you do depends on what samples you are dealing with. We
decalcify smallish samples of bone about 1x1cm - this can either compact or
trabecular bone.
Here are some of the things I learned......
1. Do not overload the chamber.....place samples between the electrodes, and
rather do fewer samples - decal can be very quick if used right.
2. Do not use metal lids on casettes if using them (or any metal clips,
parts etc)
3. If the samples aer heavily mineralised, ie huge pieces of compact bone,
change the fluid when you see that the fizzing around the electrodes has
slowed
4. Rather swich off the unit overnight (with the samples still in) if you
are doing a long decal - then swich on in the day, so that you can monitor
progress.
5. Not all sam[les will decalcify at the same rate - check them all
individually at 4 - 5hr, or in the morning of the next day
5. When decal is finished - rinse samples thoroughly in running tap water
for at LEAST and hour before processing
6. I have decalcified a slice of pig femur about 0.5 cm thick in about 5hrs

hope this helps


On Thu, Oct 1, 2009 at 7:48 PM, Isabel Cristina Soares Brandao <
isabel.brandao <@t> sarah.br> wrote:

> The time expended in the process was too long and the tissues had artifacts
> when examined on the microscope. We did exactly the same procedure described
> in the Sakura brochure.
> Do you do something different? How do you verify the process? How long does
> it take to get the samples decalcified?
>
> Thanks for your help!
>
> > ----------
> > De:   histonet-bounces <@t> lists.utsouthwestern.edu[
> SMTP:histonet-bounces <@t> lists.utsouthwestern.edu<SMTP%3Ahistonet-bounces <@t> lists.utsouthwestern.edu>]
> em nome de louise renton[SMTP:louise.renton <@t> gmail.com<SMTP%3Alouise.renton <@t> gmail.com>
> ]
> > Enviada:      quinta-feira, 1 de outubro de 2009 14:35
> > Para:         Histonet <@t> lists.utsouthwestern.edu
> > Assunto:      Re: [Histonet] Sakura TDE 30
>  >
> > Could you please explain what the problems are. I have had excellent
> results
> > with this system
> >
> > On 10/1/09, Isabel Cristina Soares Brandao <isabel.brandao <@t> sarah.br>
> wrote:
> >
> > > Hi,
> > > Does anyone out there have experience with the decalcifier System from
> > > Sakura? We have just recived the equipment but we could'nt get the
> results
> > > that they describe in the brochure.
> > >         Isabel C.S. Brandão
> > > Associação das Pioneiras Sociais
> > > Patologia Cirúrgica - Brasília - Brasil
> > >
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> >
> >
> >
> > --
> > Louise Renton
> > Bone Research Unit
> > University of the Witwatersrand
> > Johannesburg
> > South Africa
> > "There are nights when the wolves are silent and only the moon howls".
> > George Carlin
> > No trees were killed in the sending of this message.
> > However, many electrons were terribly inconvenienced.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.