[Histonet] a basic question about immunohistochemistry

[John Kiernan]

Dear Dr Inan,
 
Anyone  can fix, process and do immunohistochemistry on parts of old, dead "lab" rats. What you see will have to be compared with comparably immunostained sections of old, dead "normal" rat tissues. This is bad science!  
 
You need to repeat the experiment. Do the work yourself and don't rely on untrained or uneducated "staff".
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Salim Yalcin Inan <syinan <@t> ucalgary.ca>
Date: Friday, November 27, 2009 17:46
Subject: [Histonet] a basic question about immunohistochemistry
To: histonet <@t> lists.utsouthwestern.edu

> Dear All,
> 
> Because I am new in immunohistochemistry, I have a basic 
> question about it.
> What if your rat dies in the evening or in the weekend, which 
> you are doing
> a chronic experiment and need to collect brain tissue for
> immunohistochemistry? And let's say, the staff did not noticed 
> it to inform
> you on time. Several hours passed since your rat died. There is 
> no way to do
> perfusion. Is it still possible to do immunohistochemistry?
> Thank you very much in advance.
> 
> Best regards,
> 
> Salim Yalcin Inan, Ph.D.
> (post-doctoral fellow)
> Department of Clinical Neurosciences
> University of Calgary
> syinan <@t> ucalgary.ca
> 
> 
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