[Fwd: RE: [Histonet] Sakura Embedding]

[Victor Tobias]

Wanted to share this reply.

-------- Original Message --------
Subject: 	RE: [Histonet] Sakura Embedding
Date: 	Wed, 25 Nov 2009 09:56:56 -0500
From: 	Durden, Kelley <kelleydurden <@t> pathology.ufl.edu>
To: 	'Victor Tobias' <victor <@t> pathology.washington.edu>
References: 
<0C96F0BFE078D74C91A1C541D24A6AE4968CDA33 <@t> EMGMB1.ad.medctr.ucla.edu> 
<f8332fbe0911250047n617df137pdb17cb57e95d1ee5 <@t> mail.gmail.com> 
<4B0D4032.5040604 <@t> pathology.washington.edu>



Hey Victor,

Just went out to CA last month for Sakura training on the VIP 6 and got to look at the "dream lab" Sakura just set up.

Very cool!  At that lab they have the Automatic Embedding system.  What they do to get proper orientation at the time of grossing is they've developed special cassettes that have, for lack of a better word, baskets.  They have a biopsy cassette basket, a basket that could be used for tubular structures (ie vas deferens), they have a basket for larger specimens (uterus etc) and baskets that have rows for breast bx and prostate bx.  

The whole idea is to maintain orientation all the way through the process.  The tissues are oriented in these cassette baskets at the time of grossing.  They are loaded onto the processor, then loaded into the auto embedding center.  Never having to re orient the samples.

Then the baskets are embedded directly into the paraffin wax and are sectioned.  You section right through the basket.  It is made of a special type of plastic that is "sectionable."  It is a really cool idea and process to watch.

I brought home some samples of the baskets so I could try them here in our lab even though we don't have the auto embedding station.  We sectioned through a couple of the different basket varieties and got good results.

I'd contact my Sakura rep for some samples so you could try to section with the basket and see if it works for you.

We don't have the volume that would necessitate an auto embedder b/c we are a research lab - but if we could justify it I'd love to have it.

On another note - we love our VIP 6 processor and the training they sent me for was phenomenal.

Hope this helps!


Kelley 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victor Tobias
Sent: Wednesday, November 25, 2009 9:33 AM
To: Anne van Binsbergen
Cc: histonet <@t> lists.utsouthwestern.edu; Linke, Noelle
Subject: Re: [Histonet] Sakura Embedding

As I recall you don't have to do anything special at the time of 
embedding, but what is it like for the person grossing? They have to 
correctly orientate the tissue or the specimen could be ruined?

Victor

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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Anne van Binsbergen wrote:
> i am - love it!!
>
> 2009/11/25 Linke, Noelle <NLinke <@t> mednet.ucla.edu>
>
>   
>> Is anyone using the Sakura TissueTek AutoTec automated embedding machine in
>> their lab?  Do you like it?
>>
>> Thank you!
>> Noelle
>>
>> Noёlle Linke M.S., HTL(ASCP)QIHC
>> Manager, Histology Services
>> Department of Pathology & Laboratory Medicine
>> David Geffen School of Medicine at UCLA
>> Phone: 310-825-7397
>> Pager: 97471
>> nlinke <@t> mednet.ucla.edu<mailto:nlinke <@t> mednet.ucla.edu>
>>
>>
>>
>>
>>
>>
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-- 
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
=================================================
Privileged, confidential or patient identifiable information may be
contained in this message. This information is meant only for the use 
of the intended recipients. If you are not the intended recipient, or 
if the message has been addressed to you in error, do not read, 
disclose, reproduce, distribute, disseminate or otherwise use this 
transmission. Instead, please notify the sender by reply e-mail, and 
then destroy all copies of the message and any attachments.