[Histonet] rhodanin stain fading

[John Kiernan]

Rena is right. Published methods prescribe aqueous mounting media. This probably is the most sensitive histochemical staining method for detecting copper. See Irons et al 1977 Arch. Path. Lab. Med. 101:298-301.
 
The histochemical reagent for copper is not rhodanine (which exists but would not stain copper deposits) or rhodanin (which does not exist). It is paradimethylaminobenzylidenerhodanine. It's indexed as a D in the catalogues of vendors of chemicals. There is no short name for this reagent and technique. This is not a pedantic gripe. You need to have the right compound and also the right instructions for the technique.

John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Rena Fail <renafail <@t> bellsouth.net>
Date: Friday, November 20, 2009 18:15
Subject: Re: [Histonet] rhodanin stain fading
To: gu.lang <@t> gmx.at, histonet <@t> lists.utsouthwestern.edu

> Alcohol will cause the stain to fade, even the small amt. that 
> is carried over to the xylene over the course of  the day. Make 
> sure your xylene is fresh and coverslip immediately after staining
> Rena Fail
> 
> 
> ----- Original Message ----
> From: Gudrun Lang <gu.lang <@t> gmx.at>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Fri, November 20, 2009 2:16:49 PM
> Subject: [Histonet] rhodanin stain fading
> 
> Hi!
> 
> Yesterday I did a rhodine stain for copper. Immediatly after 
> staining I saw
> not many but distinct red granula in hepatocytes. 
> 
> I am new to this stain, so I was happy (and a little bit proud), 
> that it had
> worked.
> 
> I also stained one slide over night for comparison. Today 
> morning the first
> stained slides has faded and not the smallest bit of red colour 
> could be
> seen.
> 
> 
> 
> Is this a common problem? What causes the fast fading?
> 
> 
> 
> Gudrun
> 
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