AW: [Histonet] rhodanin stain fading

[Gudrun Lang]

Rena,
we use butylacetat for clearing and before coverslipping with Pertex
(xylolbased, resinous). Butylacetat tolerates ethanol-carryover more than
xylene, but evapourates very fast after couverslipping.
But is it possible, that butylacetat or Pertex itself cause the fading?

Do you think coverslipping with a waterbased medium is a good alternative?
Gudrun

-----Ursprüngliche Nachricht-----
Von: Rena Fail [mailto:renafail <@t> bellsouth.net] 
Gesendet: Samstag, 21. November 2009 00:15
An: gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
Betreff: Re: [Histonet] rhodanin stain fading

Alcohol will cause the stain to fade, even the small amt. that is carried
over to the xylene over the course of  the day. Make sure your xylene is
fresh and coverslip immediately after staining
Rena Fail


----- Original Message ----
From: Gudrun Lang <gu.lang <@t> gmx.at>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Fri, November 20, 2009 2:16:49 PM
Subject: [Histonet] rhodanin stain fading

Hi!

Yesterday I did a rhodine stain for copper. Immediatly after staining I saw
not many but distinct red granula in hepatocytes. 

I am new to this stain, so I was happy (and a little bit proud), that it had
worked.

I also stained one slide over night for comparison. Today morning the first
stained slides has faded and not the smallest bit of red colour could be
seen.



Is this a common problem? What causes the fast fading?



Gudrun

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