[Histonet] Re: Freezing artifacts

[Galina Deyneko]

Dear Colleagues.
Thank you very much for all answers, they are really useful. Sorry , but one more question. Some time  during cryosectioning  and mounting on glass slide (from Fisher, gold series for frozen sections)  I get bubbles underneath the tissue and these spots look very bad. I tried to use cold slide, warm slide, RT slide -no difference. How i can eliminate this problem?
Thank you in advance
Galina Deyneko.
Cambridge, MA