FW: [Histonet] ATP 9.4 AND ATP 4.3

[Ian Montgomery]

Tay,
	You'll also need pH4.6 for the acid reversal and fibre typing. In my
hands, the technique published by J.M. Round, et al, 1980. Histochem. J. 12
707-710 is the most consistent. All the solutions should be freshly prepared
and pH adjusted using a newly calibrated pH meter. The sections should be
cut just before staining. Before and after the cobalt make sure the sections
are well rinsed or you'll get 'dirty' staining from the sulphide. I also
change the bottle of sulphide regularly so buy the smallest quantity
possible and change frequently. 
	Sticking to these rules I get superb fibre typing. I've tried
numerous techniques for ATPase but always return to the Joan Round
technique.
	Any problems let me know and I'll e-mail the technique.
Ian.   

Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of tay kianlee
Sent: 18 November 2009 09:54
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ATP 9.4 AND ATP 4.3

Does anybody know tips how to do the muscle biopsy for the above tests?
Especially the preparation  for the reagents needed.
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