[Histonet] Immunohistochemistry fixation

[Walters, Katherine S]

Has anyone has any experience using immunohistochemistry with the
following fixation?
Polyethylene glycol (PEG) solution:  25% glycol, 10% ethanol (95%), 10%
formaldehyde (37%), and 55% distilled water.
Specifically if there are any enzymatic reactions that I should
avoid/block for/etc.

Thanks for any hints-I am pretty much wed to this fixative.


Katherine Walters
Histology Director
Central Microscopy Research Facilities
85 Eckstein Medical Research Building
University of Iowa
Iowa City, Iowa 52242-1101

phone: # 319-335-8142
fax:      # 319-384-4469

katherine-walters <@t> uiowa.edu
www.uiowa.edu/~cemrf