[Histonet] Hematoxylin

[Paula Pierce]

hi, 

first make sure the reagents were replaced in the correct positions if you are using an automatic stainer. Increase the time in hemat. 

I use Gill III for 10 minutes, water wash, acid alcohol- 4 dips, water wash, 1% ammonia water-1min, water wash.

Paula




________________________________
From: DELIA GARCIA <deliadfam <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, November 11, 2009 10:12:19 AM
Subject: [Histonet] Hematoxylin

I need some pointers on how to intensify the hematoxylin on the H and E stain. Is there a way to do that? I currently use Richard Allan hematoxylin 7211. The H is weak. All reagents were changed out last night. Any of your expertise would be of great help. Thank you guys in advance. 
 
Delia



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