[Histonet] Tissue falling off slides

[Weems, Joyce]

Put some alcohol in the water bath - it will reduce the surface tension.
Maybe that will help. j 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John
Shelley
Sent: Tuesday, November 10, 2009 09:25
To: histonet <@t> lists.utsouthwestern.edu
Cc: Betsy Woessner; Kathryn Gizzi
Subject: [Histonet] Tissue falling off slides

Good morning histonetters!!

I received a project from an outside source to process and embed mouse
hearts for this group. From what I could gather the specimens had
varying times in 4% paraformaldehyde from 24-48 hours all the way up to
4 months. Once processed and embedded the specimens where returned to
the group to cut. They are using superfrost plus slides from Fisherbrand
and from what I could see the sections were coming off fine. The only
thing I did notice is that when they put the slide in the water bath the
tissue seemed to be repelled which from experience means that too much
coating on slide and a hydrophobic environment is create. I asked them
to look for slides from a different lot and cut additional sections and
from those sections I asked them to air-dry only and others I asked for
them to place in oven for 30-45 mins. at 56 to 60 degrees or until they
did not see any more water droplets. They said the sections that they
placed in the oven stayed on a little longer in the deparaffinizing
steps but still fell off.  I know that it could be processing also but
the specimens looked great when I embedded them. Any suggestions will be
great, sometimes when your right on top of it you lose sight of other
possibilities.

John


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