SPAM-LOW: [Histonet] Fc Receptors

[Patsy Ruegg]

Amos,

I use fc blocker for all mouse on mouse staining, staining on sheep tissue
when my detection is made in a goat as many of them are, and in cases like
you just showed where I get non specific BG staining, I don't know if you
can make up a rule but these are my criteria.  You are right it is just too
expensive to use all the time.

Cheers,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Monday, November 09, 2009 1:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Fc Receptors

Hi,
   I have a question about Fc Receptors. I have samples that I have
IHC labelled with a few antibodies. Some were totally fine while
others had a lot of background which was eliminated when repeated with
a Fc Receptor blocker (Innovex). So my question is this: Is there a
general rule of thumb about when such a treatment would be useful?
Using it all the time would work, but aside from being unnecessary
sometimes, it would prove fairly pricey too.

Any Suggestions?
Amos

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