[Histonet] Gomori's Retic Stain

[Theresa Rohr]

Histonetters.

Suddenly having a problem with Retic stain.  Fibers weakly staining and dark silver spots deposited all over slide. 

We seem to be following same protocol we always used but I am wondering if something I am not noticing has changed.  Could it be too much Ammonium Hydroxide clearing the precipitate or too little used. Reagents made up new of course and extra care with acid glass cleaning.  Just thought maybe someone else had a similar problem and found an easy solution. 

If you have any thoughts I would be very appreciative.  

Theresa Rohr, BA, HT 
Nyack Hospital, NY
rohrt <@t> nyackhospital.org

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