[Histonet] regarding validation for the instrument install and antibodies

[Richard Cartun]

There is no straight-forward answer to question.  It really depends on how confident your pathologists are with the results of the particular IHC test.  For some proteins you may only need to run three positive and three negative cases.  For others you may need to run ten positive and ten negative cases.  Keep in mind that doing these validation studies is expensive and time-consuming.  Therefore, I always recommend that you continue to add cases (both positive and negative) prospectively (from your daily cases) to your initial validation to increase your numbers.  I also believe that laboratories should only do those IHC tests that they will perform on a regular basis, have the expertise to interpret, and the control material in-house to run in parallel with the patient specimen. 

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> <srishan <@t> mail.holyname.org> 11/3/2009 2:02 PM >>>
Hello Everyone,

Apart from ER/PR,howmany cases/control blocks need to be run for antibody 
validation?  We are talking about commonly used antibodies.

Thank you


Nirmala Srishan
Holy Name Hospital
Teaneck, NJ 07666

Holy Name Hospital  is the recipient of: 
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