[Histonet] MAP-2 antibody

[Jim Manavis]

Robert

If you are using formalin fixed paraffin embedded human material, Sigma have
a very nice mouse monoclonal that works beautifully. The cat no. is M-4403
(clone HM-2).

Cheers

Jim

Jim Manavis
Laboratory Manager
Hanson Institute
Centre for Neurological Diseases
IMVS, Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668 / 0401120697
FAX: 61-08-8222 3392
email: jim.manavis <@t> imvs.sa.gov.au
Disclaimer: Not this little black duck! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lott, Robert
Sent: Saturday, 30 May 2009 2:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Christa Hladik
Subject: [Histonet] MAP-2 antibody

Hi Everyone,

One of our pathologists is interested in MAP-2 IHC for the following
application:

 

Microtubule Associated Protein-2 Is a Sensitive Marker of Primary and
Metastatic Neuroblastoma.                         

Krishnan C, Heerema-McKenney A, Arber DA.  Department of Pathology,
Stanford University, Stanford, CA.

 

Comment: Microtubule associated protein-2 (MAP-2) is a protein expressed
in high levels in cells derived from the neural crest.  

It is a very robust marker of neuronal differentiation and has been used
most often in evaluating neuronal differentiation in tumors 

of the central nervous system.  MAP-2 showed comparable sensitivity in
staining primary neuroblastomas 

(pre- and post-treatment samples) as compared to synaptophysin,
chromogranin, CD56, and beta-catenin.  

In contrast to other markers of neuroblastoma, MAP-2 did not show
significant cross reactivity to native bone marrow 

precursors, thus eliminating a potential source of confusion.

 

Does anyone use this antibody for this or like applications....

I have looked and am "fairly" sure that there is not an IVD available,
but would still be interested in knowing which antibody you use.

 

Thanks,

 

Robert L. Lott, HTL(ASCP) / Manager, Anatomic Pathology 

Trinity Medical Center/ LabFirst/ 800 Montclair Road / Birmingham, AL
35213/ 205-592-5387 

 



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