[Histonet] Trichrome stain in GMA

[Paula Pierce]

Hello,

you are getting darker staining on the top sections because the solution is hotter at the top. You should stir the solution after heating to distribute the heat. I use a plastic transfer pipet to do this. A few draws and empties will stir the solution well.

Paula




________________________________
From: "Simoskevitz <@t> Osteotech.com" <Simoskevitz <@t> Osteotech.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Friday, May 29, 2009 12:58:39 PM
Subject: [Histonet] Trichrome stain in GMA

Does anyone have a good protocol for a Trichrome stain in glycol
methacrylate bone sections.  I keep getting non specific staining for
demineralized bone sections.  I am using a microwave technique that stains
my top section one way and my bottom section another.  I have cut at
different thicknesses tried different times and rinsing techniques.
Sometimes half of my section will stain one way while the other have stains
different.  Any help would be greatly appreciated.

Ricki

*********************************************
Ricki Simoskevitz, Research Associate III
Osteotech Inc.
51 James Way
Eatontown, NJ 07724
Phone: (732) 542-2800 X6328
Fax: (732) 935-1298
**********************************************


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