[Histonet] Sakura film coverslipper xylene residue issue

[Lynette Pavelich]

Our Sakura coverslipper is from 1994 (yep...I win the prize!!).  When we
have had those "brown" spots, we have discovered that they are actually
dry areas.  I would look at the last little white roller bar on the
track.  The springs(2) that keeps it in place, and keeps that roller bar
nice and flat to squish out the air bubbles, may be going bad.  When we
have replaced the springs, our brown spots have been eliminated.  
In addition, we have to have the volume of xylene at about 6 drops to
accomadate the cytology smears, which does leave the slides a little on
the wet side.  So we just put them under the hood for a couple minutes
to dry.

Hope this helped!  Happy Friday!!!
Lynette

>>> Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com> 05/29/09 9:52 AM >>>
I have an older Sakura coverslipper, circa 1997ish.  We have noticed an 
increasing number of brown spots on tissue sections recently.   This
does 
not occur if the same slides are covered with glass coverslips.   It
does 
not look like a drying artefact, occurs randomly throughout tissue
types, 
location on tissue, i.e., edge, center.   I've even showed these spots
to 
the rep who admitted she has never seen this artefact before.   We use 
only the Tissue Tek tape, we maintain the equipment on a regular basis. 
The xylene flow is good.   Our only problem are these random brown
spots.  
 Any suggestions?
Jackie O'



"Karin Groeger" <KGroeger <@t> USLABS.net> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/29/2009 08:19 AM

To
"Scott" <lscott <@t> sfcn.org>, <Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] Sakura film coverslipper xylene residue issue






Hi Scott,
  We place ours in front of a fan to dry if we are in a hurry, even if
we air dry them we do not have a problem with the xylene residue,
perhaps you have  a bad batch, check your lot # and call Sakura.  We
coverslip over 1,000 slides a day and have not seen this.

Karin Groeger

Histology Supervisor

US LABS, Irvine,CA

949-450-0145 ext. 649

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott
Sent: Thursday, May 28, 2009 10:03 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Sakura film coverslipper xylene residue issue

Hi,
    We have a newer Sakura film coverslipper, does anyone have
experience with this piece of equipment. I have a problem with the
slides having a light xylene residue, after the slides come off of the
coverslipper. 

How do you dry them? If I leave the slides on the coverslipper to air
dry the xylene residue is noticeable. If I lightly wipe off all of the
slides as they come off the coverslipper they look clean.

How do you guys do it? I think it is a waste of time to wipe off all of
the slides. I don't think the xylene flow is too high.

Thanks for your help,

Scott Hendricksen HT(ASCP)
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