Fw: Re: AW: [Histonet] AFB & Recycling Formalin for Fresh Tissue

[Akemi Allison-Tacha]

Hello,
I responded to Gudrun personally, but I thought that the information I am supplying him below might be a good educational source for other histologists who are having similar scenario's for AFB contamination.  

I would also like to say that I have an extremely eager histology team that have expressed an interest in learning theory and practice.  Most of my team are OJT.  They have had several pathologists approach them with these concerns regarding AFB, and because of their knowledge, or lack of it, couldn't fix the problem.  We are starting with the basics and I am giving in-services along the way.

Hi  Gudrun,

They are shipping the recycled formalin directly to the outside hospital surgery to put their fresh tissues into.....

As far as the AFB, we have had intermitant (+) AFB on the edges of tissues, that has been extremely questionable.  We currently are not cutting a negative (-) & positive(+) control on the same water bath as the test sample. That will be remedied beginning Monday. The water here is also in question.  I will be sending it out for microbacteria analysis.

We are going through a thorough housekeeping and in-service process.  We are starting with the tissue processors, which are currently only changed weekly (not rotated daily), embedding centers, which have to my knowledge, never been drained, and the hopper cleaned-out.  The water baths are not scrubbed out with soap and HOT water daily and
 covered at night to prevent contamination.  The water bath is not skimmed of excess tissue debris, each and every time another specimen is placed on it.  The Carbol Fuchsin is not being filtered prior to use.  The working solution is currently being poured back into the stock bottle.  Need I go on................

Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-tacha <@t> apmglab.com
P: E-Mail: akemiat3377 <@t> yahoo.com



--- On Thu, 5/28/09, Gudrun Lang <gu.lang <@t> gmx.at> wrote:

From: Gudrun Lang <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Recycling Formalin for Fresh Tissue
To: akemiat3377 <@t> yahoo.com
Cc:
 histonet <@t> lists.utsouthwestern.edu
Date: Thursday, May 28, 2009, 10:34 AM

Just for interest. Your lab takes the recycled formalin to make 4% neutral
buffered formalin (NBF)? Or do they ship the recycled formalin directly in
bottles to the surgery?
AFB = acid fast bacili?

Do you have concerns, that they produce false-positive AFB stains? I cannot
imagine, that a (hopefully) small number of dead bacili on the surface of
tissue will pretend this. An other question: Isn't there a filter in the
recycler to prevent such contamination?
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
akemiat3377 <@t> yahoo.com
Gesendet: Donnerstag, 28. Mai 2009 18:14
An: Histonet
Betreff: [Histonet] Recycling Formalin for Fresh Tissue

Good Morning Histo Land,

I am asking all you out there to give me your input on recycIing formalin. 
I realize this has been discussed in the not too distant past, but this may
be a little different situation.  I realize some labs are recycling formalin
to put on their tissue processors, but this is a totally different scenario.

I have been a manager at my lab for only a month.  Much to my surprise, I
just found out yesterday that our lab is recycling formalin and shipping it
out to one of our clients to put their FRESH SPECIMENS into. 
 

I just had a lengthy conversation with Robert Lott the day before yesterday,
regarding pre-analytical protocol's effecting the test results of AFB.  By
the way, we were in full agreement.  This formalin issue was unknown to me
then.  I realize that the pre-analytical, pre-analytical, pre-analytical,
process effects the final out come.  I need to present my case to our
Director of Pathology why this is compromising patient care.

Any and all responses are gladly welcome.

Thanks,
Akemi

Akemi Allison-Tacha BS, HT (ASCP) HTL
Histology Manager
Associated Pathology Medical Group Laboratories
105A Cooper Ct. Los Gatos, CA 95032
Cell: 425.941.4287 
W: E-Mail: aallison-tacha <@t> apmglab.com
P: E-Mail: akemiat3377 <@t> yahoo.com

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