AW: [Histonet] Melanin / melanocytes staining method tintorial or IHC

[Gudrun Lang]

There's a silverstain for melanin and other argentaffine granules:
Fontana-Masson.
The principle is based on the reducing effect of melanin on
silverdiaminions.
You can find the procedure in the AFIP manual.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Medea
McGraw
Gesendet: Mittwoch, 27. Mai 2009 20:36
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Melanin / melanocytes staining method tintorial or IHC

Craig,

I was searching the archives on the histonet concerning the Dopa Oxidase
stain. I have not performed this stain but have done the Schmorls stain. I
was wondering if you had a particular method/protocol that you use for this
stain? Are there any kits available or is it cheaper to do buying the
reagents separately? Any suggestions would be greatly appreciated.

Regards,
Medea


-- 
Medea J. McGraw BS, HT, HTL(ASCP) CM
IHC Research Histotechnologist
Phenopath Laboratories
Phone: 206-374-9000 x1033
Fax: 206-374-9009



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