[Histonet] RE: [IHCRG] CD21

[Tony Henwood]

Jean,
 
Here is the details from our manual
 

ANTIGEN NAME:


CD 21


 

OTHER NAMES:        

 

Clone:             2G9

Isotype:          IgG2a

 

DESCRIPTION:         Monoclonal antibody to a Prokaryotic recombinant
protein corresponding to the external domain of the CD21 molecule.

          

STORAGE CONDITIONS:        4-8oC undiluted

 

 

SUPPLIER:                Novocastra NCL-CD21-2G9 

 


PROCEDURE:


 

Methodology:        HRP-POLYMER

Working Dilution:    1/20        Bond   1/40

Special Conditions:      HIER (pH6 - Citrate)

Frozen Section: 

CONTROL TISSUE:   Lymph Node/Tonsil               

 

Inbuilt Controls                       Lymphocytes

 

 

 


CLINICAL SIGNIFICANCE:


CD21 antigen is a type I integral membrane glycoprotein of molecular
weight 140kD. The CD21 molecule, present on mature B cells, is involved
in transmitting growth-promoting signals to the interior of the B cell
and acts as a receptor for Epstein-Barr virus. The antigen is absent on
T-lymphocytes, monocytes and granulocytes.


REFERENCES:


 

Ling N R, Brown B and Hardie D (1994) Journal of Immunological Methods.
173: 11-17 

Timens W, Boes A, Vos H, et al (1991) Histochemistry. 95: 605-611 

Regards 

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


	-----Original Message-----
	From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On
Behalf Of Taylor, Jean
	Sent: Thursday, 28 May 2009 3:52 AM
	To: 'ihcrg <@t> googlegroups.com';
'histonet <@t> lists.utsouthwestern.edu'
	Subject: [IHCRG] CD21
	
	

	Hi everyone,

	 

	We are looking into ordering the CD21 antibody and was wondering
what antibody clone labs are using.

	 

	Thanks!

	 

	Jean Taylor, HT(ASCP)QIHC

	IHC Tech

	Meriter Health Services

	Madison, WI 53715


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