[Histonet] cloudy formalin in vip tissue processor

[Kemlo Rogerson]

That's a 'harsh' fixative; used it years and years ago as it mordants
the haematoxylin. I think that I still stand by the harsh effects of
that fixative on coagulating protein and that the culprit is precisely
that; coagulated protein from using a additive fixative (as you know
formalin is a coagulant, non-additive fixative, normally).



Kemlo Rogerson	
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Christine I. Braaten
Sent: 22 May 2009 17:36
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cloudy formalin in vip tissue processor

Thanks to all who have replied to my query. I probably should have
mentioned that we use zinc formalin in our processor and as our primary
fixative. I have spoken to both Sakura and Anatech (supplier of zinc
formalin) and feel more confused. Anatech says it's a change in pH that
causes the zinc to precipitate and that xylene will cause a whole lot
more problems than a little cloudiness. Sakura thinks the zinc formalin
is the problem. We clean the retort with the cleaning program every
morning and make sure to dry the retort so I'm not sure how xylene would
get into the formalin. If this brings up any other issues I would
appreciate more feedback. Thanks 


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