[Histonet] RE: microtome and processing for large specimens

[Shirley A. Powell]

Hi Gay, 
I can help you with your project.  I am doing a workshop (#40) at NSH this year on sectioning macros as well if you are going to be there.  Are you cutting human or animal, the size is important.  Some animal brains can be sectioned on regular rotary microtomes if there is enough blade clearance.  

To answer your question about microtomes Leica carries sliding microtomes which can handle large blocks/whole organ blocks.  Give me a call at 478-301-2374.  

Shirley Powell
Mercer University School of Medicine
Macon, Georgia

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gay Rudow
Sent: Friday, May 22, 2009 9:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] microtome and processing for large specimens

Hi,

We are going to start on a new project in which we will need to cut a whole paraffin-embedded brain hemisphere. Does anyone know of a company that carries a microtome made for cutting large sections? Also, if anyone has suggestions as to how to embed very large sections or how many pieces I will need to cut the brain into in order to accomplish this. Thanks for your help!
Gay
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