[Histonet] cloudy formalin in tissue processor

[Pamela Marcum]

This can result from not drying the retort or tissue tank after the clean cycle which will leave alcohol in the bottom.  Dry the tank beofre starting the next run.  Ocassionally I have even seen some xylene end up in a mixture in the tissue tank if it is not dried well.  Both of these will turn your NBF cloudy.   When you get a processor they don't always tell you to dry it to prevent carry over. 



Pam Marcum 

UPENN Vet Sch 

New Bolton Center 

----- Original Message ----- 
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com> 
To: "Christine I. Braaten" <CBraaten <@t> Cheshire-Med.COM>, histonet <@t> lists.utsouthwestern.edu, "LynnBurton" <Lynn.Burton <@t> Illinois.gov> 
Sent: Thursday, May 21, 2009 3:49:06 PM GMT -05:00 US/Canada Eastern 
Subject: RE: [Histonet] cloudy formalin in tissue processor 

If you use NBF it will get cloudy when it mixes (in some step) with alcohol. The NBF has salts that are not soluble in alcohol and will produce cloudiness. You are getting your NBF mixed with alcohol. 
René J. 

--- On Thu, 5/21/09, Burton, Lynn <Lynn.Burton <@t> Illinois.gov> wrote: 


From: Burton, Lynn <Lynn.Burton <@t> Illinois.gov> 
Subject: RE: [Histonet] cloudy formalin in tissue processor 
To: "Christine I. Braaten" <CBraaten <@t> Cheshire-Med.COM>, histonet <@t> lists.utsouthwestern.edu 
Date: Thursday, May 21, 2009, 1:23 PM 


Is your formalin picking up water from the air in the room? Check the humidity level in your room. 

Lynn Burton 
Lab Assoc. I 
Animal Disease Lab 
Galesburg, Il 61401 

________________________________ 

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Christine I. Braaten 
Sent: Thu 5/21/2009 11:41 AM 
To: histonet <@t> lists.utsouthwestern.edu 
Subject: [Histonet] cloudy formalin in tissue processor 



Has anyone had a problem with their formalin getting cloudy in their 
tissue processor? We have a VIP tissue processor and just recently 
started having problems with cloudy formalin. Any suggestions would be 
appreciated. I checked the archives and couldn't find any articles on 
this particular subject. Thanks. 


CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. 
_______________________________________________ 
Histonet mailing list 
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________ 
Histonet mailing list 
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




_______________________________________________ 
Histonet mailing list 
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet