[Histonet] Bone!

[Jack Ratliff]

I do not routinely perform decalcified bone preparations as I predominantly utilize undemineralized resin (MMA) applications, so maybe someone else can better respond to this question. However, I would say that you could store your bone in 70% EtOH until you are ready to process, but the length of time in solution storage may cause you problems down the road since you have already decalcified. I think that a prolonged storage time might make your bone hard again....if that makes any sense....and cause you a fit later when you go to the microtome. Typically, I store undemineralized bones at either 10% NBF or 70% EtOH depending upon what I care to see at the microscope.

 

Jack
 
> Date: Thu, 21 May 2009 10:46:59 -0300
> From: redward <@t> ucb.br
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Bone!
> 
> Friends,
> 
> Can someone recommend to me how to store bones (juvenile rat femur) that I have fixed and decalcified, but am not yet ready to cut (they're for vibratome, so not embedded).
> 
> Thanks!
> 
> Redward.
> 
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