[Histonet] HRP-labeled primary antibodies

[Kim Merriam]

Hi All,

Has anyone had experience doing IHC on FFPE tissues with HRP-labeled primary antibodies?  I was wondering what the best way to detect them would be.  I assume that going strait to DAB would not work, since no amplification is there.  I was thinking of using a biotinyl tyramide step to amplify the signal.

Also, do you think the final antibody concentration would need to be higher than with traditional, unlabeled primaries?

Thanks,
Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA