[Histonet] Sucrose infiltration and Bone question...
Jamie E Erickson
jamie.erickson <@t> abbott.com
Wed May 20 08:32:09 CDT 2009
I have two question about sucrose infiltration....First, I
have infiltrated many mouse brain with sucrose 30% in the past and it has
always been at 4degrees C (dc) and I'm asking myself now why? My mouse
brain are fixed in paraformaldehyde or formaldehyde for 24 hours prior so
why sucrose @ 4dc? Would it not infiltrate better (faster) at room temp?
I could not find out why 4dc...
My real problem is that I am tiring to get good mouse tibia/femur frozen
sections and I am using D-profile blade and a tape transfer method. It
worked great on my rats sections but not mouse so I think the sucrose is
not getting in to the bone. I can't use the sinking tissue as an
indicator because the bone sinks right away.
Should I go longer in sucrose (maybe 48 Hrs) but at 4dc or room temp ?
Would adding 2 % DSMO to sucrose facilitate penetration..??
Any suggestions would be great..
Thanks have a great day..
Sr. Research Associate II M.S. HTL (ASCP)
Abbott Bioresearch Center
100 Research Drive
Worcester, MA 01605-4341
e-mail: jamie.erickson <@t> abbott.com
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