[Histonet] Phenol-Formalin

[Bryan Hewlett]

Hi Bill,

Phenol-formalin fixative consists of the addition of 2% w/v phenol to 4% 
formaldehyde in phosphate buffer.
It has been recommended that sequential fixation in phenol-formalin at pH7.0 
followed by phenol-formalin at pH5.5, provides superior results.
I am attaching the reference in a separate e-mail offline.
I used this fixative extensively in the past with good results.

Regards,

Bryan


----- Original Message ----- 
From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, May 14, 2009 11:12 AM
Subject: [Histonet] Phenol-Formalin



Happy Friday!

Can anyone send me their formulation for a Penol-Formalin fixative?

Thanks.

William (Bill) O'Donnell, HT (ASCP) QIHC
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847

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