[Histonet] Toulidine blue stain

[Geoff McAuliffe]

I have always liked a very simple method from an older edition of 
Humanson's book.

1. Bring paraffin sections to water.
2. Stain in 0.1% Toluidine Blue in distilled water, 2-5 minutes
3. Rinse thoroughly in distilled water.
4. Dehydrate in 2 changes of acetone, 5 minutes each. Dehydrating in 
alcohol will wash all of the stain out.
5. Clear in xylene as usual. Use fresh xylene, not solutions containing 
carry-over alcohol from other procedures.

The second advantage of acetone dehydration is that metachromasia of 
cartilage, mast cells of some species, etc. is preserved.

Geoff

Jennifer Campbell wrote:
> Hi everyone,
>  
> I'm trying to find a protocol for a toulidine blue stain for paraffin
> sections.  I've looked in different text books and online and can't seem
> to find anything helpful.  Does anyone have a good protocol for this?  I
> need to order the stain rather soon but, first I want to know how much
> I'm going to need to run the stain.
>  
> Thanks in advance,
>  
> Jen C.
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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