[Histonet] Question about DAB waste

[Smith, Allen]

We were told that, in Miami-Dade County, we would have to apply for a license to do this.  We gave up on the cumbersome licensing procedure.
Allen A. Smith, Ph.D.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich
Sent: Wednesday, May 13, 2009 12:10 PM
To: histonet <@t> lists.utsouthwestern.edu; jcampbell <@t> vdxpathology.com
Subject: Re: [Histonet] Question about DAB waste

We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE
HISTOPATHOLOGY LABORATORY, by Dapson & Dapson, fourth edition, pg 184.

1. Prepare the following aqeous stock solutions:
    a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter)
    b.  2.0M sulfuric acid (11.2% or 112 mL concentrated acid/liter)
2. Dilute DAB solution of necessary so that its concentration does not
exceed 0.9 mg/ml.
3. For each 10ml of DAB solution, add:
    a. 5ml 0.2 M potassium permanganate (3.16% or 31.6 g/liter)
    b. 5ml 2.0 M sulfuric acid (196 g/liter, 107 ml/liter or 10.7%)
4. Allow mixture to stand for at least 10 hours.  It is now
non-mutagenic.
5. Decolorize the mixture with ascorbic acid (add powder until color
disappears).  This too is an oxidation/reduction reaction.
6. Neutralize the decolorized mixture with sodium bicarbonate (test with
pH meter, pH paper or dipsticks).  Note:  we recommend using magnesium
hydroxide/oxide instead of sodium bicarbonate.
7. Discard the solution down the drain provided that local wastewater
authorities have given their approval.

Hope this helps,
Lynette

>>> "Jennifer Campbell" <jcampbell <@t> vdxpathology.com> 05/13/09 8:51 AM
>>>
We just purchased a Dako Autostainer and are in the process of figuring
out how to get rid of the haz waste that we will be generating.  The
machine separates the haz from non-haz, thankfully.  I've been
communicating with our waste facility and have been having some
difficulty.  They want to know a lot of specifics like, how much waste
we will be brining to them at a time and how often, what sort of
containers we will be bringing it in etc.  Do you let the haz waste
carboy get to a certain level before emptying it?  Is it ok to pour this
waste into plastic containers of some sort (we were going to use empty
distilled water bottles) to transport them to the facility?  
I would appreciate any insight.  Thank you.
 
Jennifer C.
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