[Histonet] Anti -rat CD31

[anh2006 <@t> med.cornell.edu]

Clone Mec13.3 (BD Pharmingen sells it among others) works great on PFA fixed frozen tissues but you must use trypsin as an antigen retrieval step. I do 30 min at 37 deg C. Depending on your tissue that may need some adjusting.


------Original Message------
From: TF
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
To: histonet
ReplyTo: tifei <@t> foxmail.com
Sent: May 11, 2009 7:41 AM
Subject: [Histonet] Anti -rat CD31

Dear All

Just wonder whether you know any anti-rat CD31 that work on PFA-fixed tissues - frozen section ?

It seems all of them just work on acetone fixed tissues! The tissue quality can not be promised !
Even you try antigen retrieval, it wont work?

2009-05-11 



TF 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet