[Histonet] Request for Staining Advice: Rabbit Calvaria & underlying Cerebral Hemishpere

[Sean McBride]

Colleagues,

I have been charged with the task of evaluating an hydroxyapatite coated
biomaterial implanted in the calvaria of a rabbit model.

The calvaria and cerebral hemisphere were excised en bloc, and the objective
of the experiment is to assess not only new bone formation and
osseointegration but also any pathological effects on the dura mater,
arachnoid, pia mater and underlying cerebral hemisphere.

I have assessed both brain and bone individually in the past, but never en
bloc.  The specimens have been embedded in pmma and mounted for thick ground
section (~30 ­ 35 microns) evaluation.  Does anyone have any suggestions for
staining techniques that will show both bone & brain morphology in an en
bloc specimen?


Kind regards,


~Sean McBride


Senior Researcher
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-901-7540 (m)
412-268-8641 (fax)
smcbride <@t> cs.cmu.edu