[Histonet] Gram & AFB staining FNA smears

[Kemlo Rogerson]

Agree; I guess the difference is that FNAC and Micro samples haven't been subjected to processing like the tissue block sections. The tissue stains have been shown to work on something that has been fixed, dehydrated, set in hot wax, cut, rehydrated and then stained. The FNAC and Micro samples may or may not have been fixed (although I concede air drying is a form of fixation) and the stains used on them have been shown to work. 

The logic that these techniques are interchangeable is not only flawed but (oxy)moronic.





Kemlo Rogerson	
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 06 May 2009 17:24
To: histonet <@t> lists.utsouthwestern.edu; Angela Bitting
Subject: Re: [Histonet] Gram & AFB staining FNA smears

The "doc" is wrong, otherwise your histology sections to be stained with Gram & AFB should also be sent to micro. Perhaps you will not have to do them anymore. Your "doc's" reasoning is purely oxymoronic.
René J.

--- On Wed, 5/6/09, Angela Bitting <akbitting <@t> geisinger.edu> wrote:

From: Angela Bitting <akbitting <@t> geisinger.edu>
Subject: [Histonet] Gram & AFB staining FNA smears
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, May 6, 2009, 9:22 AM

The subject of staining FNA smears with AFB and Gram stains in Histology vs in Micro came up today. Is there a reason that FNAs can't be stained the same way we stain our tissue sections? One of our docs was under the impression that it's not acceptable to use the staining methods we use in our Histology lab.
I don't know what method Micro labs use, so I was hoping someone could shed some light on this subject for me.

Thanks, as always,
Angie

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
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