[Histonet] Gram & AFB staining FNA smears

mburton1 <@t> bu.edu mburton1 <@t> bu.edu
Wed May 6 18:35:36 CDT 2009


Angela,

The routine Gram stain procedure used in clinical micro labs is more 
simplified and is acceptable for staining thin smears but I don't 
believe it is suitable for tissue sections. So I would say the opposite 
is true of what the "doc" is saying: The stains used in the Histology 
lab would probably work fine for everything but I wouldn't send my 
tissue sections to the micro lab.

Mark Burton HTL ASCP
Mass General Hospital
Boston, MA 02114

Quoting Angela Bitting <akbitting <@t> geisinger.edu>:

> The subject of staining FNA smears with AFB and Gram stains in 
> Histology vs in Micro came up today. Is there a reason that FNAs 
> can't be stained the same way we stain our tissue sections? One of 
> our docs was under the impression that it's not acceptable to use the 
> staining methods we use in our Histology lab. I don't know what 
> method Micro labs use, so I was hoping someone could shed some light 
> on this subject for me.
>
> Thanks, as always,
> Angie
>
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
>
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