[Histonet] Destain Hematoxylon

[Kemlo Rogerson]

>From memory I found that it was better to give a good water wash or go
into bicarb after destaining with acid/ alcohol as the acidity impacted
on the staining if you went from acid/ alcohol directly into
haematoxylin again (if you wanted to restain it). 


 





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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Histonet
Alias
Sent: 04 May 2009 22:47
To: V. Neubert
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Destain Hematoxylon

Acid alcohol. It may not remove all of it but it will remove the
majority.

On Mon, May 4, 2009 at 5:32 AM, V. Neubert
<histonet.nospam <@t> vneubert.com>wrote:

> Hi,
>
> I want to destain hematoxylin (Shandon's Gill II). How to do that?
>
> Thanks in advance,
>
> V. Neubert
>
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--
Al Ias HT(ASCP)
Histology Manager
Pathology Laboratory
United States
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