[Histonet] re: destain hematoxylin

Matthew Close matthewtclose <@t> gmail.com
Mon May 4 14:03:13 CDT 2009


1) Acid alcohol: I use several drops of concentrated HCL in 95% ethanol (1%
HCL in 70% alcohol well too).  Simply dip your rinsed slides (or sections)
into solution a couple of times, rinse and check them... a dip in water with
about 10drops of ammonia should follow as well...
2) Saturated picric acid, followed by a couple of dips in water with several
drops of ammonia, rinse and check
3) 2% Iron alum or ferric chloride (times vary and can range from a couple
of dips to several minutes...)

-Matt Close


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