[Histonet] Re: IHC automated buffers

[PALMER Jason (SVHM)]

We also make our own TBS for our Dako autostainer, same as Jan.  I have used PBS instead of TBS at times, when transfering a bench run where I used PBS / tween (my standard buffer) to the machine.  I wonder if TBS really does give better results than TBS, when averaged out over all Abs?  I do know that TBS gets fungal contamination more easily than PBS (due to the organic / oxidisable nature of tris) and therefore has to be replaced more frequently, which is a drag!
 
Jason
 
Jason Palmer
Histology Laboratory Coordinator
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: jason.palmer <@t> svhm.org.au

Date: Mon, 30 Mar 2009 12:48:38 -0500
From: "Jan Shivers" <shive003 <@t> umn.edu>
Subject: Re: [Histonet] IHC automated buffers
To: "Charles, Roger" <rcharles <@t> state.pa.us>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <13EC8A4018C84A82A43104D961F1C2B1 <@t> auxs.umn.edu>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
        reply-type=original

We mix up our own, using Tween 20 at 0.05% per volume.  No problems here,
when using a Dako autostainer.

Jan Shivers
UMN Vet Diag Lab

----- Original Message -----
From: "Charles, Roger" <rcharles <@t> state.pa.us>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, March 30, 2009 9:53 AM
Subject: [Histonet] IHC automated buffers


Hello,
Is anyone presently making their own TBS with tween 20 buffer to be used in
automated IHC?  I would like to know if there are any problems related to
these buffers and if any deviations must be made to accommodate the
automation.

Thanks
Roger

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rcharles <@t> state.pa.us<mailto:rcharles <@t> state.pa.us>




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