[Histonet] Effects of Cytocool on IHC

Sebree Linda A LSebree <@t> uwhealth.org
Tue Mar 31 16:04:10 CDT 2009

I have heard not to use freezing spray on blocks/sections that are
destined for IHC but I never heard the reasoning behind this warning.
I've always steered clear of it when cutting for IHC and instead will
bury my block in the frost of our freezer until its good and cold.
Works fine.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Leslie
Sent: Tuesday, March 31, 2009 2:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Effects of Cytocool on IHC

In our lab many of the histotechs use Cytocool when cutting paraffin
blocks.  Has anyone heard of this having a negative effect on IHC?  


Leslie McCormack

Baptist Medical Center

Jacksonville, FL 32207

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