[Histonet] IHC automated buffers

[Jan Shivers]

We mix up our own, using Tween 20 at 0.05% per volume.  No problems here, 
when using a Dako autostainer.

Jan Shivers
UMN Vet Diag Lab

----- Original Message ----- 
From: "Charles, Roger" <rcharles <@t> state.pa.us>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, March 30, 2009 9:53 AM
Subject: [Histonet] IHC automated buffers


Hello,
Is anyone presently making their own TBS with tween 20 buffer to be used in 
automated IHC?  I would like to know if there are any problems related to 
these buffers and if any deviations must be made to accommodate the 
automation.

Thanks
Roger

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rcharles <@t> state.pa.us<mailto:rcharles <@t> state.pa.us>


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