[Histonet] Re: Vector VIP- Vector Blue- HRP &AP polymer secondary antibody

[C.M. van der Loos]

Hello Anjan Kumar,

Vector VIP, Vector Blue, yields purple and clear blue reaction products, respectively. That it self is certainly not an ideal combination for double staining, simply because these chromogens does not give much contrast. In case of co-localization there will be a problem recognizing the mixed-color from the basic colors. In this respect, Vector NovaRed and Vector Blue is a much better chromogen combination. 
What exactly do you mean with '...these chromogens come as avidin biotin conjugated enzymes...'? When working with the Labvision HRP and AP polymer secondary reagents, there is no avidin nor biotin involved. To my terminology the chromogen is the colored reaction endproduct that is visible under the microscope.

Cheers,
Chris

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands

----- Original Message -----
From: anjan kumar <drvet_anjan <@t> hotmail.com>
Date: Saturday, March 28, 2009 9:47 pm
Subject: Vector VIP- Vector Blue- HRP &AP polymer secondary antibody
To: triple immunohistochem <histonet <@t> lists.utsouthwestern.edu>, c.m.vanderloos <@t> amc.uva.nl


>  hi , 
>      I wanted to know what would happen if i use Vector Blue and 
> Vector VIP chromogen substrate with Labvision's HRP and Alkaline 
> phosphatase polymer secondary antibody. Since these chromogens come as 
> avidin biotin cojugated enzymes which may cause reaction with 
> endogenous Biotin.
>  Plz Advice.
>  
>  Dr. Anjan Kumar.K.R
>  
>  M.V.Sc Scholar
>  
>  Dept. of Veterinary Pathology
>  
>  Madras Veterinary College
>  
>  Chennai-7
>  
>  India
>  
>  email: drvet_anjan <@t> hotmail.com
>  
>  Phone: +91-9940475801
>  
>  
>  
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