[Histonet] Trypsin versus other AR-s

[Margaryan, Naira]

Hi Dears,

 

It is Friday, but not a weekend yet:-):-(

 

I just got a request from my PI to figure out:

1.      How often now day's people use Trypsin (EDTA, Proteinase K or E)
as an Antigen Retrieval for FFPE. 

2.      Why or is the Citrate Buffer pH6 more usable??? 

3.      Is Trypsin very old technique? 

 

Any educational feedback is appreciated,

Naira