[Histonet] RE: Histonet Digest, Vol 64, Issue 44
Barone, Carol
cbarone <@t> NEMOURS.ORG
Wed Mar 25 16:37:45 CDT 2009
4-12 hours dependent on sample size (a nickel if a good reference)...volume 1:20
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, March 25, 2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 64, Issue 44
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Today's Topics:
1. Re: questions re: fixing in general and Histochoice in
particular (Geoff McAuliffe)
2. Information Systems: Specimen Tracking & MiddleWare
(Michael Mihalik)
3. RE: Current books for Histotechnology (Smith, Allen)
4. Part Time Histotechnologist (Chicago, IL) (Crawford, Jennifer)
5. RE: Current books for Histotechnology (Smith, Allen)
6. RE: Current books for Histotechnology (Smith, Allen)
7. formaldehyde neutralizers (Robert Richmond)
8. Re: formaldehyde neutralizers (Rene J Buesa)
9. Histology Supervisor, Permanent Job, FREE MEDICAL BENEFITS!
(Alyssa Peterson)
10. Frozen section (Martin, Gary)
11. RE: Frozen section (McNabola, Angela)
12. Re: Current books for Histotechnology (Geoff McAuliffe)
13. RE: Frozen section (Weems, Joyce)
14. RNA and DNA yields from Laser Microdissection (Barone, Carol )
15. Re: RNA and DNA yields from Laser Microdissection
(nefff <@t> staff.uni-marburg.de)
----------------------------------------------------------------------
Message: 1
Date: Wed, 25 Mar 2009 09:35:32 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] questions re: fixing in general and
Histochoice in particular
To: Jacqui Detmar <detmar <@t> lunenfeld.ca>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49CA3324.1070308 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Jacqui Detmar wrote:
> Hi all. Having a bit of an internal debate here, so I would like to get
> the opinions of some of you in Histoland, please. Here are the
> questions:
>
>
>
> 1. When fixing with 10% NBF, for how long should you fix and what
> volume ratio of fixative:tissue should you use?
>
48 hours is a minimum according to R.D. Lillie, one week is better.
Really! Thickness of the tissue does not matter, formalin fixes slowly.
Of course, if you are doing immuno you may need to sacrifice some
fixation to retain antigenicity. Depends on the antigen.
10X the volume of the tissue.
> 2. At what temperature should one be fixing tissues?
>
Room temperature. There is more than ample scientific evidence that
fixing cold just slows down the already slow fixation process.
>
>
> Regarding Histochoice:
>
I never use proprietary fixes, you don't know what is in them and the
manufacturer can change the formulation any time he/she wants.
>
>
> 1. For how long should you fix the tissue?
>
> 2. What volume ratio of fixative:tissue should you use?
>
> 3. How long can you store Histochoice-fixed tissues in 70%
> ethanol?
>
>
>
> I think that's about it. Thanks in advance,
>
>
>
> Jacqui
>
>
>
> Jacqui Detmar, Post-doctoral Fellow
>
> Samuel Lunenfeld Research Institute
>
> Mount Sinai Hospital
>
> 25 Orde Street, room 6-1001AJ
>
> Toronto, ON M5T 3H7
>
>
>
> Email: detmar <@t> lunenfeld.ca
>
> Phone: 416-586-4800 x5607
>
> Fax: 416-586-5993
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
Geoff
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 2
Date: Wed, 25 Mar 2009 08:46:11 -0500
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: [Histonet] Information Systems: Specimen Tracking &
MiddleWare
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00c001c9ad50$13204280$3960c780$@com>
Content-Type: text/plain; charset="US-ASCII"
Good morning,
I was just at the Lab Infotech Summit in Las Vegas last week where the
subject of the conference was informatics in Anatomic and Clinical
Pathology. Along with the usual seminars were the usual vendors in the
exhibitor's hall demonstrating and talking about their products and
services.
As one of those vendors, I had the opportunity to talk to a few people and a
general trend appeared to merge -- one which I would like to dispel, if
possible.
I'd like to make sure that everyone is aware that you do NOT have to have
middleware in order to have bar coded cassettes, slides, etc., and you do
NOT have to have middleware in order to have specimen/material tracking.
Let me explain.
If, on the one hand, you are quite content with your current information
system and you simply wish to add barcodes and specimen tracking and you do
not want to work with your information system vendor because either they
don't have this capability or for some other reason, then YES, middleware is
a viable alternative.
On the other hand, if you are planning to purchase a new Information System
for your laboratory, then by all means, DEMAND of your new vendor, the
ability to have barcoded everything and to have specimen tracking built into
your new information system. There are lots of good reasons to have all
this capability in your information system and not in some middleware
product. I'd be happy to discuss the reasons for my statements, but I've
taken up enough of everyone's time. If you'd like to hear more, then
please, just ask.
I just thought everyone should know...
Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968
------------------------------
Message: 3
Date: Wed, 25 Mar 2009 09:46:22 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Current books for Histotechnology
To: 'Jennifer MacDonald' <JMacDonald <@t> mtsac.edu>
Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D53042A220D6D95B <@t> exchsrv02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald
Sent: Friday, March 20, 2009 4:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Current books for Histotechnology
Our library has funds available to purchase books for the Histotechnology
program. The problem is that we need current books. We have the latest
Bancroft and Gamble. Any other suggestions for books that are newer than
2000? I have suggested John Kiernan's latest.
By the way I did find a copy of Sheehan for $2,400!!
Jennifer MacDonald
Education Coordinator, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Wed, 25 Mar 2009 09:53:21 -0400
From: "Crawford, Jennifer" <jcrawfor <@t> aerotek.com>
Subject: [Histonet] Part Time Histotechnologist (Chicago, IL)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<571A823E0300F549BE86279A872395770283FA5B <@t> ag00-exmbx04.allegisgroup.com>
Content-Type: text/plain; charset="us-ascii"
Good morning!
I currently have a part time Histotechnologist position available in the
south suburbs of Chicago. The shifts are 10 hour days but only 1-3 days
per week are required with occasional Saturdays. ASCP certification is
required. Please contact me directly at jcrawfor <@t> aerotek.com if you or
someone you know is interested!
Best regards,
Jen
Jen Crawford, CIR
Scientific Recruiter
Aerotek Scientific Staffing
Phone: 847.221.1358
Fax: 847.303.2370
www.aerotek.com <http://www.aerotek.com>
Please do not keep me a secret...a referral is the best compliment that
I can receive!
____________________________________________________________________________________________________
This electronic mail (including any attachments) may contain information that is privileged, confidential, and/or otherwise protected from disclosure to anyone other than its intended recipient(s). Any dissemination or use of this electronic email or its contents (including any attachments) by persons other than the intended recipient(s) is strictly prohibited. If you have received this message in error, please notify us immediately by reply email so that we may correct our internal records. Please then delete the original message (including any attachments) in its entirety. Thank you.
------------------------------
Message: 5
Date: Wed, 25 Mar 2009 09:55:51 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Current books for Histotechnology
To: 'Jennifer MacDonald' <JMacDonald <@t> mtsac.edu>
Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D53042A220D6D974 <@t> exchsrv02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
Definitely get the 4th edition of Kiernan. Also get the latest edition of Polak and van Noorden's INTRODUCTION TO IMMUNOCYTOCHEMISTRY. If you don't already have them, used second or third editions of Lillie's HISTOPATHOLOGIC TECHNIC AND PRACTICAL HISTOCHEMISTRY and Pierce's HISTOCHEMISTRY, THEORETICAL AND APPLIED are surprisingly useful.
-Allen A. Smith,Ph.D.
Barry University School of Podiatric Medicine
Miami Shores, Florida
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald
Sent: Friday, March 20, 2009 4:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Current books for Histotechnology
Our library has funds available to purchase books for the Histotechnology
program. The problem is that we need current books. We have the latest
Bancroft and Gamble. Any other suggestions for books that are newer than
2000? I have suggested John Kiernan's latest.
By the way I did find a copy of Sheehan for $2,400!!
Jennifer MacDonald
Education Coordinator, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Wed, 25 Mar 2009 10:01:53 -0400
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] Current books for Histotechnology
To: 'Ingles Claire ' <CIngles <@t> uwhealth.org>
Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D53042A220D6D989 <@t> exchsrv02.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"
For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore.
-Allen a. Smith,Ph.D.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, March 23, 2009 11:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Current books for Histotechnology
I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing.
Claire
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Jennifer MacDonald
Sent: Fri 3/20/2009 3:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Current books for Histotechnology
Our library has funds available to purchase books for the Histotechnology
program. The problem is that we need current books. We have the latest
Bancroft and Gamble. Any other suggestions for books that are newer than
2000? I have suggested John Kiernan's latest.
By the way I did find a copy of Sheehan for $2,400!!
Jennifer MacDonald
Education Coordinator, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald <@t> mtsac.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 25 Mar 2009 10:06:03 -0400
From: Robert Richmond <RSRICHMOND <@t> aol.com>
Subject: [Histonet] formaldehyde neutralizers
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60903250706p50a524c0ic88b4c1f734e529a <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Thanks, Tony Henwood! Your explanation of how to neutralize
formaldehyde with ammonia is the only clear explanation of
formaldehyde neutralization I've ever read.
One more question: how does neutralization with sodium bisulfite work?
What are the advantages and disadvantages of the two methods?
Bob Richmond
Samurai Pathologist
Knoxville, Tennessee
------------------------------
Message: 8
Date: Wed, 25 Mar 2009 07:38:45 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] formaldehyde neutralizers
To: histonet <@t> lists.utsouthwestern.edu, Robert Richmond
<RSRICHMOND <@t> aol.com>
Message-ID: <925805.52259.qm <@t> web65705.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Me too!
René J.
--- On Wed, 3/25/09, Robert Richmond <RSRICHMOND <@t> aol.com> wrote:
From: Robert Richmond <RSRICHMOND <@t> aol.com>
Subject: [Histonet] formaldehyde neutralizers
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, March 25, 2009, 10:06 AM
Thanks, Tony Henwood! Your explanation of how to neutralize
formaldehyde with ammonia is the only clear explanation of
formaldehyde neutralization I've ever read.
One more question: how does neutralization with sodium bisulfite work?
What are the advantages and disadvantages of the two methods?
Bob Richmond
Samurai Pathologist
Knoxville, Tennessee
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Wed, 25 Mar 2009 10:39:29 -0400
From: Alyssa Peterson <alyssa <@t> alliedsearchpartners.com>
Subject: [Histonet] Histology Supervisor, Permanent Job, FREE MEDICAL
BENEFITS!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bbc6db3a0903250739i41b932b9mceec0e395e640964 <@t> mail.gmail.com>
Content-Type: text/plain; charset=windows-1252
*Position of the Histology Supervisor*
Do you want the benefits you deserve with the rewards you can see?
Do you want the advancement, flexibility and resources to advance your
career and provide the care that your patients need?
**FREE medical and dental benefits, domestic partner benefits, excellent
tuition reimbursement, continuing education and more!**
*Description:*
Supervise, coordinate and participate in providing laboratory services to
meet the needs of patients as ordered by the medical staff and performed in
accordance with defined standards and practices in general and unique to
assigned section(s).
*Day shift with variable start times - Monday - Friday*
Bachelor of Science degree in Medical Technology or related biological
science
Six years of medical lab experience of which two years must be in particular
section supervised.
NYS licensure required
HT, (ASCP) preferred
*Montgomery**, **NY** area:*
*60 miles from Danbury, CT*
*60 miles from Newark, NJ*
*60 miles from Scranton, PA*
What a great opportunity! REMEMBER, THIS WON'T LAST. Interested? Please send
resume in Microsoft Word format to: Alyssa <@t> alliedsearchpartners.com
**Please forward this email to anyone who you seems fit for this position,
as the referral bonus for this position is $1000 if we place a person that
you send to us in a position!**
--
Alyssa Peterson
Allied Search Partners
O: 770.621.2639 ext. 4
F: 770.621.2640
------------------------------
Message: 10
Date: Wed, 25 Mar 2009 07:42:44 -0700
From: "Martin, Gary" <gmartin <@t> marshallmedical.org>
Subject: [Histonet] Frozen section
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6ED9D4252F278841A0593D3D788AF24C04DD56D4 <@t> mailsvr.MARSHMED.local>
Content-Type: text/plain; charset="us-ascii"
We need to make a change in the way we presently account for our frozen
section while doing them. Presently we receive the requisition with the
first specimen, then pathology is responsible to account for any
subsequent specimens. The problem is that the subsequent specimens are
typically labeled poorly, and we are trying very hard to conform to the
CAP guidelines. So ... when the specimens are not labeled in detail, it
requires follow up calls to gain the proper information. I would like
to know how other facilities are handling multiple frozen sections.
Thanks
Gary
------------------------------
Message: 11
Date: Wed, 25 Mar 2009 10:50:08 -0400
From: "McNabola, Angela" <lamcna <@t> bpthosp.org>
Subject: RE: [Histonet] Frozen section
To: "Martin, Gary" <gmartin <@t> marshallmedical.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6FAFE015E5AC6B4ABAEF2883A6F8A6D9016C6393 <@t> EXCH1.bpthosp.org>
Content-Type: text/plain; charset="us-ascii"
We require a form for each specimen. Yes, the subsequent forms may have
less information (i.e. procedure being performed), but all have the
patient id information, sample type and description, etc. They are
treated as separate samples.
-Angela
Angela McNabola, MS,HT(ASCP)SLS, QIHC
Manager Histology/Cytology
Department of Pathology
Bridgeport Hospital
267 Grant Street
Bridgeport, CT 06610
phone: 203-384-4434
lamcna <@t> bpthosp.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Gary
Sent: Wednesday, March 25, 2009 10:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen section
We need to make a change in the way we presently account for our frozen
section while doing them. Presently we receive the requisition with the
first specimen, then pathology is responsible to account for any
subsequent specimens. The problem is that the subsequent specimens are
typically labeled poorly, and we are trying very hard to conform to the
CAP guidelines. So ... when the specimens are not labeled in detail, it
requires follow up calls to gain the proper information. I would like
to know how other facilities are handling multiple frozen sections.
Thanks
Gary
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 25 Mar 2009 10:55:13 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Current books for Histotechnology
Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>, 'Ingles Claire '
<CIngles <@t> uwhealth.org>
Message-ID: <49CA45D1.4060903 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
A for atlases, I also like Wheater's FUNCTIONAL HISTOLOGY, or Gartner
and Hiatt's COLOR ATLAS OF HISTOLOGY. I don't like Ross and Pawlina's
HISTOLOGY, the
third edition by Ross, Romrell and Kaye is much better and might be cheaper.
Geoff
Smith, Allen wrote:
> For an atlas, Ross and Pawlina's HISTOLOGY, Wheater's FUNCTIONAL HISTOLOGY, or Gartner and Hiatt's COLOR ATLAS OF HISTOLOGY are all good. They are also more accurate than DiFiore.
> -Allen a. Smith,Ph.D.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles Claire
> Sent: Monday, March 23, 2009 11:55 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Current books for Histotechnology
>
> I would strongly recommend di Fiore's Atlas of Histology by Victor P. Eroschenko. I don't know what edition its in now. I used it when I went through my program. It is great for microscopic anatomy, especially when combined with actual slide viewing.
> Claire
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Jennifer MacDonald
> Sent: Fri 3/20/2009 3:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Current books for Histotechnology
>
>
>
> Our library has funds available to purchase books for the Histotechnology
> program. The problem is that we need current books. We have the latest
> Bancroft and Gamble. Any other suggestions for books that are newer than
> 2000? I have suggested John Kiernan's latest.
> By the way I did find a copy of Sheehan for $2,400!!
>
> Jennifer MacDonald
> Education Coordinator, Histotechnician Training Program
> Mt. San Antonio College
> 1100 N. Grand Ave.
> Walnut, CA 91789
> (909) 594-5611 ext. 4884
> jmacdonald <@t> mtsac.edu
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 13
Date: Wed, 25 Mar 2009 11:40:34 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Frozen section
To: "Martin, Gary" <gmartin <@t> marshallmedical.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5D64396A0D4A5346BEBC759022AAEAA53EADC3 <@t> ITSSSXM01V6.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We require a separate req for each.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
Gary
Sent: Wednesday, March 25, 2009 10:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen section
We need to make a change in the way we presently account for our frozen
section while doing them. Presently we receive the requisition with the
first specimen, then pathology is responsible to account for any
subsequent specimens. The problem is that the subsequent specimens are
typically labeled poorly, and we are trying very hard to conform to the
CAP guidelines. So ... when the specimens are not labeled in detail, it
requires follow up calls to gain the proper information. I would like
to know how other facilities are handling multiple frozen sections.
Thanks
Gary
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 14
Date: Wed, 25 Mar 2009 11:46:03 -0400
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] RNA and DNA yields from Laser Microdissection
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<37E4BAC017F57141AF64FAA5AEB04CE801471BC3 <@t> wlmmsx01.nemours.org>
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Histonet: I have a couple questions I would like to throw out to the experts:
1. Do you think higher yields of DNA /RNA in LMD are related to protocol, method differences from one instrument to another ( i.e. catapult verses,, gravity drop, verses,
arcturus melt system...or more related to histologist v. research tech handling of the sample?
2. How do you feel about frozen verses paraffin as related to yield and quality in LMD/LCM?
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Message: 15
Date: Wed, 25 Mar 2009 17:58:20 +0100
From: nefff <@t> staff.uni-marburg.de
Subject: Re: [Histonet] RNA and DNA yields from Laser Microdissection
To: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Cc: histonet <@t> lists.utsouthwestern.edu
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<20090325175820.h7xmiauajo48kgw0 <@t> home.staff.uni-marburg.de>
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Hi Carol!
My experience is, the yield depends on the protocol you use. I've done
the LCM system from arcturus and the PALM-System from Zeiss. The
highest yields had been for RNA and DNA by using the Pico Pure Kit
(MSD) while doing LCM. This had been frozen sections. We isolated DNA
from FFPE-Tissue from PALM and LCM by a conventional Proteinase K
digest and phenol extraction.
There is a paper of Fend and Specht at the Am. J. of Pathology, 2002
or 2003 describing these protocols.
As long as the histopathologist or the tech are able to identify the
"cell/tissue" of interest, it doesn't influence the yield/quality of
the RNA/DNA.
Frauke
Zitat von "Barone, Carol " <cbarone <@t> NEMOURS.ORG>:
> Histonet: I have a couple questions I would like to throw out to the experts:
>
> 1. Do you think higher yields of DNA /RNA in LMD are related to
> protocol, method differences from one instrument to another ( i.e.
> catapult verses,, gravity drop, verses,
> arcturus melt system...or more related to histologist v. research
> tech handling of the sample?
>
> 2. How do you feel about frozen verses paraffin as related to yield
> and quality in LMD/LCM?
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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