[Histonet] hematoxylin Question

[Swain, Frances L]

What I do Kristen if I use it infrequently is I get a large brown bottle, label it with working hematoxylin.  After I use the hematoxylin for whatever I am using it for I pour it in the brown bottle, cap it tightly and store it on the shelf or in a cabinet.  Whenever I need it again I get it out, set up a filtering stand with a funnel, filtering paper etc and filter it back into the dish to be used again.  You can test your hematoxylin to see if it is still good by dropping a drop in either distilled water or 37% formaldehyde.  It is turns blue purple it is still good if it turns a mauve color toss it.

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Tuesday, March 24, 2009 9:44 AM
To: histonet
Subject: [Histonet] hematoxylin Question

Hello All,
How long is hematoxylin (we use Richard-Allen Heme 2) good for after it is poured into a staining container?  The stain gets used so infrequently I would like to keep it as long as I can.  We currently dump it weekly regardless of use.  Thank you.
 
-Kristen


      
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